If a peripheral blood smear is reviewed, platelets will be observed attached to WBCs. Obtain a sample in ammonium oxalate, and count platelets utilizing a hemocytometer grid, if available, as per described methods [ 4 ]. Retrieved from ” https: It is a rare condition that occurs when an IgG antibody forms in the presence of EDTA, the anticoagulant that is used for the collection of hematology blood specimens. Platelet Function Testing , Specimen Management. As the antibody coats the platelets, the platelets rosette around segmented neutrophils, bands, and sometimes around monocytes. Journal List Case Rep Hematol v.
If the instruments’ platelet count of a sample is within or above normal range, a count may be given with an added comment noting the presence of platelet clumps and suggesting that the true count is likely higher than reported. Please help to improve this article by introducing more precise citations. No items in your cart. Thank you for your question, and as always, I invite comments from our participants. Aug 31, 1: The count will probably be flagged by the analyzer for platelet clumps or giant platelets. Clinical Presentation A year-old female presented to our hospital with complaint of abdominal pain.
Obtain a sample in ammonium oxalate, and count platelets clumpef a hemocytometer grid, if available, as per described methods [ 4 ]. If clumping persists, continue to Step 3. Recommendations From a practical laboratory point of view, investigation of platelet clumping may include the following steps until a nonclumping smear is obtained, noting that Steps 3 and 4 are reserved for the rare instances, where Steps 1 and 2 do not resolve the platelet clumping.
It showed that EDTA-dependent PTCP is a phenomenon related to the presence of natural autoantibodies with antiplatelet activity and is not associated with any pathological significance [ 4 ]. This plateelets observation may help further separate the two conditions presumptively upon examination of PB smear; refer to Figure 2 for morphologic comparison and Table 2 for comparative features. Aug 31, 1: Pseudothrombocytopenia or platelet clumping as a possible cause of low platelet count in patients with viral infection: The incidence of pseudothrombocytopenia reported in different studies ranges from 0.
Pseudothrombocytopenia – Wikipedia
No items in your cart. A photomicrograph of her stained PB smear is provided in Figure 1. The use of an alternate anticoagulant, such as citrate or heparin, may be helpful. The Journal of Pediatrics.
Herein, we discuss a case of a year-old girl who was found to have platelet clumping and discuss the work-up she underwent to investigate her pseudothrombocytopenia. Views Read Edit View history. Pseudothrombocytopenia or spurious thrombocytopenia is an in-vitro sampling problem which may mislead the diagnosis towards the more critical condition of thrombocytopenia.
The platelet count from a tube that contains liquid sodium citrate will need to be corrected for the dilutional effect of the citrate. In the case of satellitosis, collect the platelet count specimen in a sodium citrate tube. Sign up for LabCE’s mailing list. It is often, but not always, a phenomenon commonly caused by the anticoagulant EDTA. Aug 31, Home Products Most Popular Contact. The phenomenon was not age-related or gender-related, nor was it associated with any particular pathology or use of specific drugs.
It is a rare condition that occurs when an IgG antibody forms in the presence of EDTA, the anticoagulant that is used for the collection of hematology blood specimens.
CBC showed thrombocytopenia, baseline coagulation profile was normal, and PB smear showed platelet clumping.
Retrieved from ” https: If Step 3 is not possible, proceed to Step 4. Reporting platelet counts on samples that show platelet clumps can be a challenge. This can be accomplished by multiplying the platelet count that is obtained from the automated analyzer by 1.
If either platelet satellitism or platelet clumping is observed on the peripheral smear, the sample could be recollected using sodium citrate as the anticoagulant. Log In Sign up for our mailing list. Repeat testing of the new sample presumed to be collected in heparin showed normal platelet count.
Platelet changes | eClinpath
Physical examination was unremarkable. The latter is an in vivo consumption of platelet, which results in true thrombocytopenia. Articles lacking in-text citations from July All articles lacking in-text citations.
A recommended approach is not to give a platelet result value for a platwlets with clumps if the instrument’s count is below the lower limits of normal, report the clumping, and recommend one of the steps described above. Retrieved 12 May Platelet satellitism was first reported in the early ‘s.
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A pseudothrombocytopenia false-positive result may occur when automated platelet counting devices are used. Platelet clumping can also occur in the presence of EDTA blpod the platelet count again will be falsely decreased.
It is a relatively uncommon phenomenon caused by in vitro agglutination of platelets. Open in a separate window. In some labs we gently vortex the specimen and repeat the CBC with blood film exam, however the recommended approach is to collect a fresh specimen, bloos the mixing protocol.
The phenomenon occurs when the anticoagulant used while testing the blood sample causes clumping of platelets which mimics a low platelet count.
A year-old female presented to our hospital with complaint plqtelets abdominal pain. This page was last edited on 10 Juneat Capillary collections are prone to clotting and formation of platelet clumps. Online laboratory continuing education for clinical laboratories and med techs.
By Director Dennis Ernst. The image on the right illustrates platelet satellitism with platelets adhering cumped a neutrophil. In satellitosis, the platelet count usually drops to below the lower limit of the reference interval and would be flagged by the instrument.
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The patient presented with extensive bruising. Journal List Case Rep Hematol v. The authors declare that there are no competing interests regarding the publication of this paper.